Plasmid_Backbone
Part:BBa_K4361105:Design
Designed by: Lars van den Biggelaar Group: iGEM22_TUDelft (2022-09-07)
pET-11a
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal XbaI site found at 5594
Illegal PstI site found at 1068 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal NheI site found at 5639
Illegal PstI site found at 1068 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal BglII site found at 5528
Illegal BamHI site found at 5672 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal XbaI site found at 5594
Illegal PstI site found at 1068 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 316
Illegal XbaI site found at 5594
Illegal PstI site found at 1068
Illegal NgoMIV site found at 3394
Illegal NgoMIV site found at 3748
Illegal NgoMIV site found at 3908
Illegal NgoMIV site found at 5496 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1242
Illegal SapI.rc site found at 2324
Design Notes
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Source
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